The "heinous murder" occurred on Saturday, said Eugene Owusu, the UN's humanitarian chief in South Sudan. The UN did not say who the victims were or what aid agency they worked for.
They were traveling between Juba, the capital, and the town of Pibor. At least 79 aid workers have been killed in South Sudan since the conflict started in December 2013, the UN says. "These attacks against aid workers and aid assets are utterly reprehensible," said Mr Owusu.
"They not only put the lives of aid workers at risk, they also threaten the lives of thousands of South Sudanese who rely on our assistance for their survival." He called on "all those in positions of power" to stop such attacks and end "the impunity that has prevailed".
Two other attacks have targeted aid workers this month, according to the UN. A humanitarian convoy was attacked while responding to a cholera outbreak in Yirol East on 14 March, with one health worker and one patient killed.
On 10 March, local staff of an international aid organisation were detained by armed rebels during fighting in Mayendit town for four days before being released.
